SARS-CoV-2 (COVID-19) S1 protein, His Tag
SARS-CoV-2 S1 protein, His Tag (S1N-C52H2) is expressed from human 293 cells (HEK293) .
Product Specifications
Background
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 110-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
QHD43416.1
Expression Region
Val 16 - Arg 685
Host
HEK293
Target
Spike S1
Conjugation
Unconjugated
Tag
C-10xHis
Source
SARS-CoV-2
Stability
● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 12 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H2) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.2-6 ng/mL (QC tested).
Format
Liquid
Buffer
10 mM PB, 50 mM NaCl, pH7.4
Molecular Weight
76.9 kDa
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
200ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/S1N-C52H2.pdf
Species
SARS-CoV-2
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