Human GCGR / Glucagon receptor Protein, His Tag (MALS verified)

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.9 kDa. The protein migrates as 28-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P47871-1

Expression Region

Ala 26 - Lys 136

Host

HEK293

Target

GCGR

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Human GCGR, His Tag (Cat. No. GCR-H52H3) at 1 μg/mL (100 μL/well) can bind Human Anti-GCGR (Volagidemab), Human IgG2 with a linear range of 0.1-4 ng/mL (QC tested).