Biotinylated Human FGL1 Protein, His, Avitag™ (recommended for biopanning)

Fibrinogen-like protein 1 (FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1. The protective effect of fibrinogen-like protein 1 (FGL1) in liver injury has previously been reported. However, studies have shown that FGL1 may be a predictor of GC patients and a target for GC therapy. Immunocytochemical studies revealed that fgl1 selectively binds to defective spermatozoa in the cauda epididymidis. Northern blot analysis and in situ hybridization demonstrated the high expression of fgl1 in the principal cells of the proximal cauda epididymidis. Immunofluorescence analysis using mouse fibrotic lung tissues suggested that fibrotic regions showed increased expressions of Gtse1 and Fgl1, Gtse1 and Fgl1 are suggested to be novel targets for radiation-induced lung fibrosis.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 37.9 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q08830-1

Expression Region

Leu 23 - Ile 312

Host

HEK293

Target

FGL1

Conjugation

Biotin-labeled

Tag

N-10xHis & N-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate can bind Anti-FGL1 antibody with a linear range of 1-40 ng/mL (QC tested).