Human CCL5/RANTES Protein, Fc Tag (MALS verified)

C-C chemokine ligand 5 (CCL5), also known as RANTES, is a target gene of NF-kappaB, and is expressed by T lymphocytes, macrophages, platelets, synovial fibroblasts, tubular epithelium, and certain types of tumour cells. CCL5 plays a role in recruiting a variety of leukocytes into inflammatory sites including T cells, macrophages, eosinophils, and basophils. CCL5 activity is mediated through its binding to CCR1, CCR3, and mainly CCR5.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.4 kDa. The protein migrates as 40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P13501-1

Expression Region

Ser 24 - Ser 91

Host

HEK293

Target

CCL5

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human CXCL4, His Tag (Cat. No. CX4-H52H9) at 5 μg/mL (100 μL/well) can bind Human CCL5, Fc Tag (Cat. No. CC5-H5251) with a linear range of 0.156-2.5 μg/mL (QC tested).