Recombinant Streptavidin Protein (MALS verified)
Recombinant Streptavidin (STN-N5116) is expressed from E.coli cells.
Product Specifications
Background
Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI) .Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
CAS Number
9000-83-3
Specifications
This protein carries no "tag". The protein has a calculated MW of 13.8 kDa.
Expression Region
Ala 37 - Ser 163
Host
E. coli
Target
Streptavidin
Conjugation
Unconjugated
Tag
-
Source
Streptomyces avidinii
Applications
ELISA assay
Stability
-20°C to -70°C for 2 years in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).
Format
Powder
Buffer
NaCl, pH7.4
Additives
Trehalose
Molecular Weight
13.8 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
1mg*5
Host or Source
E. coli
Species
Streptomyces avidinii
Preservative
Trehalose
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