Human IL-12 R beta 2 Protein, His Tag (MALS verified)

The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12RB2 is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. IL-12B2 gene acts as tumor suppressor in human acute and chronic B-cell leukemias/lymphomas, IL-12R beta 2 directly restrains multiple myeloma cell growth, and targeting of IL-12 to tumor cells holds promise as new therapeutic strategy.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.0 kDa. The protein migrates as 85-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q99665-1

Expression Region

Lys 24 - Asn 622

Host

HEK293

Target

IL-12 R beta 2

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human IL-12 R beta 2, His Tag (Cat. No. ILB-H52H6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer, His,Avitag&Flag Tag (Cat. No. IL2-H8210) with a linear range of 0.01-0.3 μg/mL (QC tested).