Monkeypox virus (strain Zaire-96-I-16) H3L Protein, His Tag (MALS verified)

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. H3L is similar to Vaccinia virus strain Copenhagen H3L heparin binding surface protein (Cop-H3L) surface membrane protein. There are research reports H3L induced transcriptional perturbations and injuries.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 34.6 kDa. The protein migrates as 35-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q8V4Z2

Expression Region

Met 1 - Phe 282

Host

HEK293

Target

H3L (MPXV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Monkeypox virus (strain Zaire-96-I-16)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag (Cat. No. H3L-M52H1) at 1 μg/mL (100 μL/well) can bind Anti-Monkeypox virus/MPXV H3L Antibody with a linear range of 4-63 ng/mL (QC tested).