Human KRAS (G12A) Protein, His Tag (active enzyme)
Human KRAS (G12A), His Tag (KRS-H51H1) is expressed from E. coli cells. It contains AA Thr 2 - Cys 185 (G12A) (Accession # P01116-2 (G12A) ) .
Product Specifications
Background
KRAS (Kirsten rat sarcoma 2 viral oncogene homolog) gene is a proto-oncogene that encodes a small GTPase transductor protein called KRAS. KRAS is also known as Ki-Ras, c-K-ras and c-Ki-ras. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation, promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. RAS is one of the most frequently mutated oncogenes in human cancer but the frequency and distribution of RAS gene mutations are not uniform. In details, mutation of glycine 12 (G12) causes RAS activation by interfering with GAP binding and GAP-stimulated GTP hydrolysis. The reference shows the pathway may as a potential therapy targets.
Specifications
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 22.9 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) .
Accession Number
P01116-2 (G12A)
Expression Region
Thr 2 - Cys 185 (G12A)
Host
E. coli
Target
KRAS
Conjugation
Unconjugated
Tag
N-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Format
Powder
Buffer
50 mM Tris, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
22.9 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/KRS-H51H1.pdf
Species
Human
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