Human KRAS (G12A) Protein, His Tag (active enzyme)

KRAS (Kirsten rat sarcoma 2 viral oncogene homolog) gene is a proto-oncogene that encodes a small GTPase transductor protein called KRAS. KRAS is also known as Ki-Ras, c-K-ras and c-Ki-ras. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation, promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. RAS is one of the most frequently mutated oncogenes in human cancer but the frequency and distribution of RAS gene mutations are not uniform. In details, mutation of glycine 12 (G12) causes RAS activation by interfering with GAP binding and GAP-stimulated GTP hydrolysis. The reference shows the pathway may as a potential therapy targets.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 22.9 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) .

Accession Number

P01116-2 (G12A)

Expression Region

Thr 2 - Cys 185 (G12A)

Host

E. coli

Target

KRAS

Conjugation

Unconjugated

Tag

N-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.