Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein (Peptide free, MALS verified)
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein (HL1-H82E5) is expressed from human 293 cells (HEK293) . It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*04:01) (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI9239961.1 (HLA-DRB1*04:01) ) .
Product Specifications
Specifications
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 29.2 kDa and 28.8 kDa. The protein migrates as 35-40 kDa and 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
CAI2388006.1 (HLA-DRA1*01:01) & CAI9239961.1 (HLA-DRB1*04:01)
Expression Region
Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*04:01)
Host
HEK293
Target
HLA-DRA1*0101 & HLA-DRB1*0401
Conjugation
Biotin-labeled
Tag
C-10xHis & C-Avi
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein (Cat. No. HL1-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-DR Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
29.2 kDa and 28.8 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
25ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/HL1-H82E5.pdf
Species
Human
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