Biotinylated Human IL-5 R alpha / CD125 Protein, Avitag™, Fc Tag (MALS verified)

Interleukin-5 receptor subunit alpha (IL5RA) is also known as IL-5 receptor subunit alpha, IL-5R subunit alpha and CD125, which is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and type 5 subfamily.IL5R, a heterodimer of an alpha and a beta subunit, is expressed on eosinophils and basophils. IL5RA / CD125 is the receptor for interleukin-5 (IL5) and the alpha chain can bind to IL5. The WSXWS motif of IL5RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The beta subunit of IL5R is common to the IL3, IL5 and GM-CSF receptors.

Product Specifications

Background

Specifications

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag. The protein has a calculated MW of 64.9 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q01344-1

Expression Region

Asp 21 - Trp 342

Host

HEK293

Target

IL-5 R alpha

Conjugation

Biotin-labeled

Tag

N-Avi & N-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-5, His Tag, premium grade (Cat. No. IL5-H52H3) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag (Cat. No. ILA-H82F5) with a linear range of 0.1-10 ng/mL (QC tested).