Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Monomer, MALS verified)

Ovalbumin (OVA) can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. ovalbumin (Ova) was presented to T cells if it was released from pinosomes into the cytosol by osmotic lysis of pinosomes. Immunization with the adjuvanted SIINFEKL peptide induces long-lasting CD8+ T cell immunity in mice.

Product Specifications

Background

Specifications

Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 39.7 kDa and 15.7 kDa. The protein migrates as 48-53 kDa and 17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P01901 (H-2Kb) & P01887 (B2M) & SIINFEKL

Expression Region

Gly 22 - Thr 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide

Host

HEK293

Target

H-2Kb & B2M & OVA (SIINFEKL)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Cat. No. H2A-M82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind anti-H-2Kb/OVA (SIINFEKL) antibody with a linear range of 0.1-4 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

39.7 kDa and 15.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Mouse

Protein ID

P01901 (H-2Kb) & P01887 (B2M) & SIINFEKL

Preservative

Trehalose

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