FITC-Labeled Human CD5 Protein, Fc Tag (MALS verified)

T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1, which is phosphorylated on tyrosine residues by LYN, so CD5 can create binding sites for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development, selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 65.1 kDa. The protein migrates as 75-95 kDa and >116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P06127-1

Expression Region

Arg 25 - Pro 372

Host

HEK293

Target

CD5

Conjugation

FITC Labeled

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized FITC-Labeled Human CD5, Fc Tag (Cat. No. CD5-HF255) at 1 μg/mL (100 μL/well) can bind Mouse Anti-CD5 Antibody, Mouse IgG1 with a linear range of 0.2-3 ng/mL (QC tested).