Biotinylated Human immunodeficiency virus 1 gp140 Protein, His, Avitag™ (MALS verified)

The trimeric envelope glycoproteins (Env) that are displayed on human and simian immunodeficiency viruses (HIV and SIV, respectively) are heterodimers of the transmembrane glycoprotein (gp41) and a surface glycoprotein (gp120) . The glycoproteins gp120 and gp41 are synthesized initially as a single gp160 polypeptide that is subsequently cleaved to generate the noncovalently associated gp120/gp41 complex. Soluble versions of trimeric gp140, either cleaved or uncleaved, are being developed as immunogens to elicit a protective humoral immune response against HIV-1 infection.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 53.8 kDa & 20.9 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

ABA61516.1

Expression Region

Ala 30 - Asp 661

Host

HEK293

Target

Envelope glycoprotein gp140/gp140 (HIV)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human immunodeficiency virus 1 (HIV-1)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human immunodeficiency virus 1 gp140 Protein, His,Avitag (Cat. No. GP0-V82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259) with a linear range of 0.1-2 ng/mL (QC tested).