Biotinylated Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1

Mertansine (DM-1) is a tubulin inhibitor that binds to the ends of microtubules and inhibits microtubule dynamics. DM-1 (Mertansine) has antitumor activity and functions as a regulator of tubulin. It is an alpha-amino acid ester, a carbamate, an epoxide, an organic heterocyclic tetracyclic compound, an organochlorine compound, a mercaptan, and a maydenin alkaloid. DM-1, derived from Mydenin, is a cytotoxic component of antibody-drug conjugations that produce antibody-drug conjugations via a sulfhydryl group splice with SPP (n-succinimide 4- (2-pyridyl dithio) ) or SMCC (4- (3-mercapto-2, 5-dioxy-1 pyrrolidyl) -cyclohexanic acid) splice.

Product Specifications

Background

CAS Number

9007-83-4

Host

Hybridoma

Origin Species

Mouse

Target

DM-1

Conjugation

Biotin-labeled

Tag

Mouse IgG1 | Mouse Kappa

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.

Purity

90%

Bioactivity

Immobilized Human Her2 / ErbB2 (498-648) Protein, His Tag (Cat. No. HE2-H52H4) at 2 μg/mL, add increasing concentrations of Trastuzumab-DM1 (T-DM1), and then add Biotinylated Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (MALS verified) (Cat. No. DM1-BLY73) at 0.5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 1.95 ng/mL (Routiney tested).