Anti-Phospho-p27 (S178) CDKN1B Antibody

Boster Bio Anti-Phospho-p27 (S178) CDKN1B Antibody catalog # A00173S178. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27

Gene Name

CDKN1B

UniProt

P46527

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Ser178. AA range:144-193

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Applications

IHC, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Form

Liquid

Function

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995) . Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A- CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Molecular Weight

22073 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Fragment

IgG

Other Gene Names

Cyclin-dependent kinase inhibitor 1B

Subcellular Location

Nucleus. Cytoplasm. Endosome . Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity) . .