Anti-Phospho-Rb (S608) RB1 Antibody

Boster Bio Anti-Phospho-Rb (S608) RB1 Antibody catalog # A00039S608. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1;

Gene Name

RB1

UniProt

P06400

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser608. AA range:581-630

Clonality

Polyclonal

Tissue Specificity

Expressed in the retina.

Applications

WB, IHC, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Form

Liquid

Function

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity) .