Anti-LRRC32 Purified Monoclonal Antibody

Boster Bio Anti-LRRC32 Purified Monoclonal Antibody (Catalog# M08199) . Tested in WB application (s) . This antibody reacts with Human.

Product Specifications

Background

GARP (glycoprotein A repetitions predominant protein), also known as garpin or LRRC32 (leucin-rich repeat containing protein 32) is an approximately 80 kDa transmembrane glycoprotein detected on the surface of megakaryocytes, platelets, and activated Treg cells. It binds to the latency-associated peptide (LAP) domain of pro-TGF beta and regulates its storage and activation. The expression of GARP on Treg cells seems to be necessary for their suppressive functions.

Synonyms

GARP, D11S833E

Gene Name

LRRC32

UniProt

Q14392

Host

Mouse

Reactivity

Human

Immunogen

Purified human sGARP protein. The mouse monoclonal antibody GARP5 recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.

Clonality

Monoclonal

Clone

Clone: GARP5

Tissue Specificity

Preferentially expressed in regulatory T-cells (Tregs) .

Applications

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Dilution

Western blotting: 1-2 μg/ml; positive control: human thrombocytes, reducing conditions

Form

Liquid

Function

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed:19750484, PubMed:19651619, PubMed:22278742) . Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed:22278742) . Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta (PubMed:22278742) . Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) (PubMed:19750484, PubMed:19651619) . Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity) .