Anti-Ms CD49e Purified Itga5 Monoclonal Antibody

Boster Bio Anti-Ms CD49e Purified Itga5 Monoclonal Antibody (Catalog# M01911-1) . Tested in ICC, Flow Cytometry, IHC-F application (s) . This antibody reacts with Mouse.

Product Specifications

Background

CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.

Synonyms

ITGA5, VLA-5 alpha, FNRA

Gene Name

Itga5

UniProt

P11688

Host

Rat

Reactivity

Mouse

Immunogen

C57BL/6 x A/J) F1 murine mast cell line. The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.

Clonality

Monoclonal

Clone

Clone: 5H10-27 (MFR5)

Applications

ICC, Flow Cytometry, IHC-F

Purification

Purified by protein-G affinity chromatography.

Concentration

1 mg/ml

Dilution

Flow cytometry: 1-4 μg/ml.

Form

Liquid

Function

Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling.