Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody

Boster Bio Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (Catalog# M03134-1) . Tested in Flow Cytometry, IP, WB, ICC, ELISA application (s) . This antibody reacts with Rat, Cow, Mouse, Human, Pig.

Product Specifications

Background

Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.

Synonyms

CHC, CLHC, MRD56

Gene Name

CTLC

UniProt

Q00610

Host

Mouse

Reactivity

Rat, Cow, Mouse, Human, Pig

Immunogen

The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.

Clonality

Monoclonal

Clone

Clone: BF-06

Applications

Flow Cytometry, IP, WB, ICC, ELISA

Purification

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .

Concentration

1 mg/ml

Dilution

Flow cytometry: 2-5 μg/ml; positive control: human blood leukocytes. Intracellular staining.

Form

Liquid

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch- TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter- microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582) . The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825) . Plays a role in early autophagosome formation (PubMed:20639872) .