Anti-Hu CD300a Purified Monoclonal Antibody

Boster Bio Anti-Hu CD300a Purified Monoclonal Antibody (Catalog# M05953) . Tested in Flow Cytometry, WB application (s) . This antibody reacts with Human.

Product Specifications

Background

CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.

Synonyms

IRC, CLM-8, IGSF12, CMRF35H

Gene Name

CD300A

UniProt

Q9UGN4

Host

Mouse

Reactivity

Human

Immunogen

HPB human acute lymphoid leukemia cell line. The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

Clonality

Monoclonal

Clone

Clone: MEM-260

Tissue Specificity

Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.

Applications

Flow Cytometry, WB

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Form

Liquid

Function

Inhibitory receptor which may contribute to the down- regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed:10746781, PubMed:16339535, PubMed:9701027) . Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed:22043923) .