Anti-Hu CD45 Purified PTPRC Monoclonal Antibody

Boster Bio Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (Catalog# M00555-3) . Tested in Flow Cytometry, IP, WB, IHC-P, ICC application (s) . This antibody reacts with Human.

Product Specifications

Background

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

Synonyms

LCA, T200, LY5, B220, GP180, TPC

Gene Name

PTPRC

UniProt

P08575

Host

Mouse

Reactivity

Human

Immunogen

Human thymocytes and T lymphocytes. The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

Clonality

Monoclonal

Clone

Clone: MEM-28

Tissue Specificity

Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.

Applications

Flow Cytometry, IP, WB, IHC-P, ICC

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Form

Liquid

Function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity) .