Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody

Boster Bio Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (Catalog# M09548-1) . Tested in Flow Cytometry, WB, IHC-P, IHC-F, ICC application (s) . This antibody reacts with Human.

Product Specifications

Background

CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.

Synonyms

HNK1, LEU7, 3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc

Gene Name

B3GAT1

UniProt

Q9P2W7

Host

Mouse

Reactivity

Human

Immunogen

A pool of neuroblastoma cell lines. The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.

Clonality

Monoclonal

Clone

Clone: TB01

Tissue Specificity

Mainly expressed in the brain.

Applications

Flow Cytometry, WB, IHC-P, IHC-F, ICC

Purification

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .

Concentration

1 mg/ml

Form

Liquid

Function

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.