Anti-VISTA VSIR Antibody

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5) .

VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54

VISTA antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human VISTA. The immunogen is located within the last 60 amino acids of VISTA.

Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level) . Expressed at high levels in placenta, spleen, plasma blood leukocytes, and lung. Expressed at moderate levels in lymph node, bone marrow, fat, uterus, and trachea. Has low expression levels in other tissues.

Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993) . May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity) . May stimulate MMP14-mediated MMP2 activation (PubMed:20666777) .

VISTA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.