Anti-DIS3-like exonuclease 2 DIS3L2 Antibody

The exosome is involved in a multitude of cellular RNA processing and degradation events (1) . DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species (2) . DIS3L2 is a paralog of DIS3, but unlike DIS3 is located in the cytoplasm (3) . DIS3L2 defines a novel eukaryotic RNA degradation pathway and preferentially targets uridylated RNA (4) . DIS3L2 mutations have also been associated with sporadic Wilms tumors (3) .

DIS3L2 antibody is human, mouse and rat reactive. At least three isoforms of DIS3L2 are known to exist; this antibody will only detect the longest isoform. DIS3L2 is predicted to not cross-react with DIS3 or DIS3L.

DIS3L2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DIS3L2. The immunogen is located within amino acids 720 - 770 of DIS3L2.

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.