Anti-LRRC8A Antibody

Boster Bio Anti-LRRC8A Antibody (Catalog # A03517) . Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Background

The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2) . LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1) . LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3) .

Synonyms

Leucine rich repeat containing 8 family member A, leucine-rich repeat-containing protein 8A, LRRC8, AGM5

Gene Name

LRRC8A

UniProt

Q8IWT6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

LRRC8A antibody is human, mouse and rat reactive.

Immunogen

LRRC8A antibody was raised against a 15 amino acid peptide near the amino terminus of human LRRC8A. The immunogen is located within amino acids 60 - 110 of LRRC8A.

Clonality

Polyclonal

Tissue Specificity

Expressed in brain, kidney, ovary, lung, liver, heart, and fetal brain and liver. Found at high levels in bone marrow; lower levels are detected in peripheral blood cells. Expressed on T-cells as well as on B-lineage cells.

Applications

ELISA, WB, IHC-P

Field of Research

Stem Cells, Signal Transduction

Purification

LRRC8A antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24725410, PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731, PubMed:29769723) . The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24725410, PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731) . Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed:28193731) . LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471) . Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E) ; channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658, PubMed:28193731) . Can form functional channels by itself (in vitro) (PubMed:26824658) . Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746) . Also required for T-cell development (By similarity) .