Anti-IZUMO1 Antibody

Boster Bio Anti-IZUMO1 Antibody (Catalog # A02772) . Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.

Product Specifications

Background

IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2) . IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3) . Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4) .

Synonyms

Izumo sperm-egg fusion 1, IZUMO, Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo

Gene Name

IZUMO1

UniProt

Q8IYV9

Host

Rabbit

Reactivity

Human

Cross Reactivity

IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms.

Immunogen

IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1. The immunogen is located within amino acids 170 - 220 of IZUMO1.

Clonality

Polyclonal

Tissue Specificity

Sperm-specific (at protein level) . Detectable on sperm surface only after the acrosome reaction.

Applications

ELISA, WB, IHC-P

Field of Research

Stem Cells

Purification

IZUMO1 antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.