Anti-Transcription factor Spi-B SPIB Antibody

Boster Bio Anti-Transcription factor Spi-B SPIB Antibody (Catalog # A02805-1) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Background

SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1) . SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3) . It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN) -producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4) .

Synonyms

Spi-B transcription factor, SPI-B

Gene Name

SPIB

UniProt

Q01892

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.

Immunogen

SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB. The immunogen is located within amino acids 200 - 250 of SPIB.

Clonality

Polyclonal

Tissue Specificity

Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Stem Cells, Immunology

Purification

SPIB antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN) -producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.