Anti-IQSEC1 Antibody

ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1) . As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3) . IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4) .

IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.

IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1. The immunogen is located within amino acids 60 - 110 of IQSEC1.

Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.

Guanine nucleotide exchange factor for ARF1 and ARF6 (PubMed:24058294) . Guanine nucleotide exchange factor activity is enhanced by lipid binding (PubMed:24058294) . Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.