Anti-Neuroligin-2 NLGN2 Antibody

Neuroligin 2 (NLGN2) is a member of a family of neuronal cell surface proteins that localize to the post-synaptic membrane (1,2) . Neuroligins are thought to act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses (2) . NLGN2 has been shown to control perisomatic inhibitory synapse maturation together with gephyrin and collybistin, thereby regulating GABA receptor clustering on neurons (3,4) . NLGN2 also exhibits differential functions at different types of inhibitory synapses on the same postsynaptic neuron (5) .

NLGN2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NLGN2 antibody is predicted to not cross-react with other members of the NLGN protein family.

NLGN2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human NLGN2. The immunogen is located within amino acids 630 - 680 of NLGN2.

Expressed in the blood vessel walls. Detected in colon, brain and pancreas islets of Langerhans (at protein level) . Detected in brain, and at lower levels in pancreas islet beta cells.

Transmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Plays a role in synapse function and synaptic signal transmission, especially via gamma- aminobutyric acid receptors (GABA (A) receptors) . Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Promotes clustering of postsynaptic LHFPL4 (By similarity) . Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion (By similarity) .

Cell membrane ; Single-pass type I membrane protein . Cell junction, synapse, postsynaptic cell membrane . Cell junction, synapse, presynaptic cell membrane . Detected at postsynaptic membranes in brain. Detected at dendritic spines in cultured neurons. Colocalizes with GPHN and ARHGEF9 at neuronal cell membranes (By similarity) . Localized at presynaptic membranes in retina. Colocalizes with GABRG2 at inhibitory synapses in the retina (By similarity) . .