Anti-Rubicon RUBCN Antibody
Boster Bio Anti-Rubicon RUBCN Antibody (Catalog # A12453) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Product Specifications
Background
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1) . Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2) . Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2) .
Synonyms
Run domain Beclin-1 interacting and cysteine-rich containing protein, KIAA0226
Gene Name
KIAA0226
UniProt
Q92622
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
Immunogen
Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon. The immunogen is located within amino acids 240 - 290 of Rubicon.
Clonality
Polyclonal
Applications
ELISA, WB, IHC-P, IF
Field of Research
Autophagy
Purification
Rubicon antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL
Form
Liquid
Function
Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745) .
Molecular Weight
108622 MW
Shipping Conditions
Available
Storage Conditions
Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Fragment
IgG
Other Gene Names
KIAA0226
Subcellular Location
Late endosome
Protein Name
KIAA0226
Frequently Asked Questions
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