Anti-DOCK8 Antibody

The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1) . DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2) . Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3) .

DOCK8 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DOCK8. The immunogen is located within the last 50 amino acids of DOCK8.

Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:28028151, PubMed:22461490) . During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity) . Required for CD4 (+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151) . Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed:25762780) .