Anti-TRIM33 Antibody

Boster Bio Anti-TRIM33 Antibody (Catalog # A03133) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Background

TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2) . The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3) . TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3) . It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4) . It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5) .

Synonyms

ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7

Gene Name

TRIM33

UniProt

Q9UPN9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.

Immunogen

TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33. The immunogen is located within amino acids 580 - 630 of TRIM33.

Clonality

Polyclonal

Tissue Specificity

Expressed in stem cells at the bottom of the crypts of the colon (at protein level) . Expressed in colon adenomas and adenocarcinomas (at protein level) . Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Stem Cells

Purification

TRIM33 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity) . Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade) .