Anti-Protein crumbs homolog 2 CRB2 Antibody

CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.

At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.

Rabbit polyclonal CRB2 antibody was raised against a 17 amino acid peptide near the center of human CRB2. The immunogen is located within amino acids 460 - 510 of CRB2.

Expressed in retina, fetal eye and brain. Also expressed in kidney, ARPE-19 and RPE/choroid cell lines, and at low levels in lung, placenta, and heart.

Apical polarity protein that plays a central role during the epithelial-to-mesenchymal transition (EMT) at gastrulation, when newly specified mesodermal cells move inside the embryo. Acts by promoting cell ingression, the process by which cells leave the epithelial epiblast and move inside the embryo to form a new tissue layer. The anisotropic distribution of CRB2 and MYH10/myosin-IIB at cell edges define which cells will ingress: cells with high apical CRB2 are probably extruded from the epiblast by neighboring cells with high levels of apical MYH10/myosin-IIB. Also required for maintenance of the apical polarity complex during development of the cortex.