Anti-DDX60 Antibody

Boster Bio Anti-DDX60 Antibody (Catalog # A09938) . Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Background

DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.

Synonyms

Probable ATP-dependent RNA helicase DDX60, DEAD box protein 60

Gene Name

DDX60

UniProt

Q8IY21

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.

Immunogen

DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60. The immunogen is located within amino acids 1140 - 1190 of DDX60.

Clonality

Polyclonal

Tissue Specificity

Brain, lymph node, prostate, stomach, thyroid, tongue, trachea, uterus, skeletal muscle, spleen, kidney, liver and small intestine.

Applications

ELISA, WB, IF

Field of Research

Innate Immunity

Purification

DDX60 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Positively regulates DDX58/RIG-I- and IFIH1/MDA5- dependent type I interferon and interferon inducible gene expression in response to viral infection. Binds ssRNA, dsRNA and dsDNA and can promote the binding of DDX58/RIG-I to dsRNA. Exhibits antiviral activity against hepatitis C virus and vesicular stomatitis virus (VSV) .

Molecular Weight

197853 MW

Shipping Conditions

Available

Storage Conditions

DDX60 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.