Anti-Ras-related protein Rab-21 RAB21 Antibody

RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER) -like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α -tail of endocytosed integrin.

RAB21 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human RAB21. The immunogen is located within the last 50 amino acids of RAB21.

Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.

Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general (By similarity) . As a result, may regulate cell adhesion and migration (By similarity) . During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis (PubMed:18804435) . Involved in neurite growth (By similarity) . Following SBF2/MTMT13-mediated activation in response to starvation-induced autophagy, binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion (PubMed:25648148) .

RAB21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.