Anti-AFAP1L AFAP1L1 Antibody

The actin filament-associated protein 1 (AFAP1) -like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation (1, 4) . Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.

AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1. The immunogen is located within amino acids 70 - 120 of AFAP1L.

Expressed in breast, colon and brain. In all 3 tissues, expressed in the microvasculature (at protein level) . In addition, in the breast, found in the contractile myoepithelial cell layer which surrounds the breast ducts (at protein level) . In the colon, expressed in the mucous membrane and colonic crypts and in the smooth muscle cell layer which provide movement of the colon (at protein level) . In the cerebellum, localized around the Purkinje neurons and the granule cells of the granular layer, but not inside cell bodies (at protein level) . Outside of the cerebellar cortex, expressed in glial cells (at protein level) . Highly expressed away from the cell bodies within the dentate nucleus (at protein level) .

AFAP1L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.