Anti-TYW2 TRMT12 Antibody

TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.

TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms

TYW2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2. The immunogen is located within the first 50 amino acids of TYW2.

S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha- carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86.

TYW2 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.