Anti-ZFP219 ZNF219 Antibody

ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.

ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219. The immunogen is located within amino acids 90 - 140 of ZFP219.

Transcriptional regulator (PubMed:14621294, PubMed:19549071) . Recognizes and binds 2 copies of the core DNA sequence motif 5'-GGGGG-3' (PubMed:14621294) . Binds to the HMGN1 promoter and may repress HMGN1 expression (PubMed:14621294) . Regulates SNCA expression in primary cortical neurons (PubMed:19549071) . Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with SOX9 (By similarity) . Plays a role in chondrocyte differentiation (By similarity) .

ZFP219 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.