Anti-NOD3 NLRC3 Antibody

NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3), is predominantly expressed in the immune system, particularly in T lymphocytes, and its expression is strongly down-regulated following stimulation of the T-cell receptor complex and CD28, suggesting that NOD3 plays a role in attenuating the activation of T cells. NOD3 inhibits NF-kappaB, AP-1 and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or treatment with PMA/ionomycin and decreases IL-2 and CD25 mRNA induction in activated cells.

NOD3 antibody was raised against an 18 amino acid synthetic peptide near the center of human NOD3. The immunogen is located within amino acids 630 - 680 of NOD3.

Negative regulator of the innate immune response (PubMed:15705585, PubMed:22863753, PubMed:25277106) . Attenuates signaling pathways activated by Toll-like receptors (TLRs) and the DNA sensor STING/TMEM173 in response to pathogen-associated molecular patterns, such as intracellular poly (dA:dT), but not poly (I:C), or in response to DNA virus infection, including that of Herpes simplex virus 1 (HSV1) (By similarity) (PubMed:22863753) . May affect TLR4 signaling by acting at the level of TRAF6 ubiquitination, decreasing the activating 'Lys-63'- linked ubiquitination and leaving unchanged the degradative 'Lys- 48'-linked ubiquitination (PubMed:22863753) . Inhibits the PI3K- AKT-mTOR pathway possibly by directly interacting with the posphatidylinositol 3-kinase regulatory subunit p85 (PIK3R1/PIK3R2) and disrupting the association between PIK3R1/PIK3R2 and the catalytic subunit p110 (PIK3CA/PIK3CB/PIK3CD) and reducing PIK3R1/PIK3R2 activation. Via its regulation of the PI3K-AKT-mTOR pathway, controls cell proliferation, predominantly in intestinal epithelial cells (By similarity) . May also affect NOD1- or NOD2-mediated NF-kappa-B activation (PubMed:25277106) . Might also affect the inflammatory response by preventing NLRP3 inflammasome formation, CASP1 cleavage and IL1B maturation (PubMed:25277106) .

NOD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.