Anti-Autophagy-related protein 101 ATG101 Antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy.

ATG101 antibody was raised against a 16 amino acid synthetic peptide near the center of human ATG101. The immunogen is located within amino acids 80 - 130 of ATG101.

ATG101 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.