Anti-CCDC134 Antibody

The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.

CCDC134 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CCDC134. The immunogen is located within the first 50 amino acids of CCDC134.

Expressed in cervical gland, cervical squamous epithelium, endometrium, stomach, kidney distal convoluted tubule, spermatogenic cells in testis, mammary gland, liver and striated muscle (at protein level) (PubMed:18087676, PubMed:23070808) . Also detected in placenta (PubMed:18087676) . Highest expression in testis relative to other tissues (PubMed:18087676) . Detected in T cells and dendritic cells; highly expressed in activated CD8 (+) T cells, and also expressed at lower levels in CD4 (+) T cells (PubMed:25125657) .

In extracellular secreted form, promotes proliferation and activation of CD8 (+) T cells, suggesting a cytokine-like function (PubMed:25125657) . Enhances cytotoxic anti-tumor activity of CD8 (+) T cells (PubMed:25125657) . May inhibit ERK and JNK signaling activity (PubMed:18087676, PubMed:23070808) . May suppress cell migration and invasion activity, via its effects on ERK and JNK signaling (PubMed:23070808) .

CCDC134 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.