Anti-Protocadherin-12 PCDH12 Antibody

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2, 289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling.

PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12. The immunogen is located within amino acids 30 - 80 of PCDH12.

Expressed in highly vascularized tissues including the heart and placenta, but most tissues contain a low level of expression (PubMed:11063261) . Prominent expression in the spleen (PubMed:11063261) . Present in villous and extravillous trophoblast (at protein level) (PubMed:21402705) .

Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions (By similarity) . Acts as a regulator of cell migration, probably via increasing cell-cell adhesion (PubMed:21402705) . Promotes homotypic calcium-dependent aggregation and adhesion and clusters at intercellular junctions (By similarity) . Unable to bind to catenins, weakly associates with the cytoskeleton (By similarity) .

PCDH12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.