Anti-PHAP III ANP32E Antibody

Boster Bio Anti-PHAP III ANP32E Antibody (Catalog # A07456) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Background

Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Synonyms

LANPL, LANP-L, Acidic leucine-rich nuclear phosphoprotein 32 family member E, LANP-like protein

Gene Name

ANP32E

UniProt

Q9BTT0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

PHAP III has no cross-reaction to PHAP I and PHAP I2a.

Immunogen

PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III. The immunogen is located within amino acids 200 - 250 of PHAP III.

Clonality

Polyclonal

Tissue Specificity

Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification

PHAP III Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Histone chaperone that specifically mediates the genome- wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511) . Inhibits activity of protein phosphatase 2A (PP2A) . Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.