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Native Pseudomonas sp. Protocatechuate 3, 4-dioxygenase: Powder. 3.0 U/mg

In enzymology, a protocatechuate 3,4-dioxygenase (EC 1.13.11.3) is an enzyme that catalyzes the chemical reaction: 3,4-dihydroxybenzoate + O2 ↔ 3-carboxy-cis, cis-muconate. Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate (protocatechuic acid) and O2, whereas its product is 3-carboxy-cis, cis-muconate. This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases) . This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, iron.

Product Specifications

CAS Number

9029-47-4

Applications

This enzyme is useful for enzymatic determination of choline esterase when coupled with p-hydroxybenzoate hydroxylase.

Activity

GradeIII 3.0U/mg-solid or more (containing approx. 40% of stabilizers)

Storage Conditions

-20°C

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