Human BMP-4 Protein

Bone morphogenetic protein -4 (BMP-4) is one of nine structurally related BMP's, belonging to transforming growth factor-β (TGF-β) superfamily, which secretes proteins. Mature BMP-4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP-4 treatment promoted the proliferation of HCC cells and induced autophagy. Mechanism study shows that autophagy induced by BMP-4 is mediated by activating JNK1/Bcl2 pathway. JNK1 inhibitor and JNK1 knockout can weaken BMP-4-induced autophagy and eliminate BMP-4-promoted HCC cell growth. BMP-4 promotes the proliferation of HCC through autophagy activation of JNK1/Bcl-2 signaling.

Product Specifications

Background

Bone morphogenetic protein-4 (BMP-4) is one of nine structurally related BMP's, belonging to transforming growth factor-β (TGF-β) superfamily, which secretes proteins. Mature BMP-4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP-4 treatment promoted the proliferation of HCC cells and induced autophagy. Mechanism study shows that autophagy induced by BMP-4 is mediated by activating JNK1/Bcl2 pathway. JNK1 inhibitor and JNK1 knockout can weaken BMP-4-induced autophagy and eliminate BMP-4-promoted HCC cell growth. BMP-4 promotes the proliferation of HCC through autophagy activation of JNK1/Bcl-2 signaling

Product Name Alternative

Bone morphogenetic protein 4; BMP2B

Gene ID

652

Accession Number

P12644

Tag

BMP-4

Type

Cytokines and proteins

Applications

No tag

Purity

> 95 % as determined by SDS-PAGE

Activity

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is 1.205 ng/mL.

Molecular Weight

11.9 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized recombinant Human BMP-4 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant Human BMP-4 consists of 106 amino acids and predicts a molecular mass of 11.9 kDa

Formulation

Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 8.0.

Applications Notes

Human

Other Product Names

Bone morphogenetic protein 4; BMP2B

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human BMP-4 protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.