Mouse β-NGF protein, His tag (Animal-Free)

Beta-Nerve Growth Factors (Beta-NGF) is a 27 kDa cytokine with 241 amino acid residues. Beta-NGF belongs to neurotrophin family, and acts as neurotrophic factors. It's composed of alpha, beta, gamma subnuits, and the beta subunit is related to its biological activity. Beta-NGF binds to p75 neurotrophin receptor and Trk receptor and their function is about cell death and survival, respectively.

Product Specifications

Background

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of Rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and Rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species

Product Name Alternative

β-Nerve Growth Factor, NGF-β

Accession Number

P01139

Tag

β-NGF

Type

Cytokines and proteins

Applications

His tag

Purity

>98% as determined by SDS-PAGE.

Activity

Measure by its ability to induce TF-1 cells proliferation.The ED₅₀ for this effect is <1ng/mL.The specific activity of recombinant mouse beta-NGF is > 1 x 10⁶ IU/mg.

Molecular Weight

The protein has a calculated MW of 14.41 kDa. The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis) .

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The protein has a calculated MW of 14.41 kDa.The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis) .

Formulation

The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. If you have any concerns or special requirements, please confirm with us.

Applications Notes

Mouse

Other Product Names

β-Nerve Growth Factor, NGF-β

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse β-NGF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously