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Mouse CNTF Protein, His tag (Animal-Free)

Ciliary Neurotrophic Factor (CNTF) is the member of IL-6 cytokine family and mainly expressed in the nervous system. Mouse CNTF shares 84% sequence homology with human CNTF. CNTF is 22.9 kDa neurotrophic factor containing 110 residues, which shows multiple effects in vertebrate retinogenesis. Besides, CNTF acts as a promoter that not only accelerates adult neurogenesis but also increases the survival of neuron after injury.

Product Specifications

Background

Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. Its actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracellular CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks

Product Name Alternative

AI429687

Accession Number

P51642

Tag

CNTF

Type

Cytokines and proteins

Applications

His tag

Purity

>98% as determined by SDS-PAGE.

Activity

Testing in progress

Molecular Weight

The protein has a calculated MW of 23.40 kDa.The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis) .

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Mouse CNTF consists of 198 amino acids and predicts a molecular mass of 22.6 kDa

Formulation

The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us

Applications Notes

Mouse

Other Product Names

AI429687

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse CNTF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

Preparation

E. coli

AA Sequence

Amino acid sequence derived from Mouse CNTF (Ala2-Met198) (P51642) was expressed with 6xHis tag at the C-terminus

Available Sizes

Frequently Asked Questions

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