Human BMP-2 Protein, His tag (Animal-Free)

Bone Morphogenetic Protein-2 (BMP-2) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-2 can bind with the TGF-β receptor to active SMAD protein signal transduction. Moreover, it engages in the path of signals such as Wnt and Notch. BMP-2 plays an essential role in the growth of hard bones and cartilage and involved in the differentiation of osteoblasts.

Product Specifications

Background

Product Name Alternative

Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

Accession Number

P12643

Tag

BMP-2

Type

Cytokines and proteins

Applications

His tag

Purity

>95% as determined by SDS-PAGE.

Activity

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <1.0 μg/mL.

Molecular Weight

The protein has a calculated MW of 13.76 kDa. The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis) .

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Human BMP-2consists of 115 amino acids and predicts a molecular mass of 13 kDa

Formulation

The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us.

Applications Notes

Human

Other Product Names

Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human BMP-2 Protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously

Preparation

E. coli

AA Sequence

Amino acid sequence derived from Human BMP-2 (Gln283-Arg396) (P12643) was expressed with 6xHis tag at the C-terminus

Available Sizes

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