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Human VEGF165 protein, His tag (Animal-Free)

Vascular Endothelial Growth Factors 165 (VEGF165) is a potent growth and angiogenic cytokine which belongs to the VEGF family, includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Human VEGF165 is an abundant glycosylated cytokine composed of two identical 165 amino acid chains. Human VEGF165 plays an important role in embryonic vasculogenesis, angiogenesis and neurogenesis.

Product Specifications

Background

VEGF165, also known as Vascular Endothelial Growth Factor 165, is a protein that plays a critical role in angiogenesis, the process of forming new blood vessels from pre-existing ones. It belongs to the vascular endothelial growth factor family and is one of the most well-studied isoforms of VEGF. Human VEGF165 protein is a crucial regulator of angiogenesis, with implications in various physiological and pathological processes. Its study contributes to our understanding of blood vessel development, tissue repair, and the development of therapeutic strategies for diseases such as cancer

Product Name Alternative

VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen

Accession Number

NP_001165097

Tag

VEGF165

Type

Cytokines and proteins

Applications

His tag

Purity

>98% as determined by SDS-PAGE.

Activity

Measure by its ability to induce HUVEC cells proliferation.The ED₅₀ for this effect is <5 ng/mL.The specific activity of recombinant human VEGF165 is approximately >1.4 x 10⁶ IU/mg.

Molecular Weight

The protein has a calculated MW of 20.11 kDa. The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis) .

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized Human VEGF165 protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles.

Protein Length

The Human VEGF165 protein consists of 189 amino acids and has a predicted molecular mass of 20.1 kDa

Formulation

Lyophilized from sterile PBS, pH 8.0

Applications Notes

Human

Other Product Names

VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously

Preparation

E. coli

AA Sequence

Amino acid sequence derived from Human VEGF165 protein (Ala207-Arg395) (NP_001165097) was expressed with 6xHis tag at the C-terminus

Available Sizes

Frequently Asked Questions

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